TABLE 3

Precision by concentration and serotypea

SerotypeConcnLog10 titerbTiterbSD% CVc
Repeatability varianceBetween-run varianceIntermediate precisionRepeatability varianceBetween-run varianceIntermediate precision
O1A13.5843,8410.0400.0550.0689.2312.6715.72
O1A22.9829600.0710.0950.11916.5022.0827.80
O1A32.3802400.0390.0390.0559.058.9312.74
O1A41.778600.0490.0920.10411.2321.5224.39
O1A51.176150.0460.1320.14010.6731.1733.11
O1AOverall 0.0580.0780.09713.4018.0122.58
O213.7175,2080.0710.0000.07116.380.00016.38
O223.0181,0420.0660.0800.10415.3418.6324.30
O232.3192080.0760.1080.13217.6725.2231.12
O241.620420.0510.0000.05111.780.00011.78
O2Overall 0.0820.0510.09618.9611.8022.44
O6A13.1641,4580.0550.0070.05612.751.6012.85
O6A22.6874860.0530.0520.07412.2711.9717.21
O6A32.2091620.0460.0150.04810.523.5411.11
O6A41.732540.0350.0370.0518.098.6211.84
O6A51.255180.0490.0270.05611.336.1412.91
O6AOverall 0.0480.0330.05811.017.5213.36
O25B12.5023170.0580.0560.08113.5212.8718.75
O25B22.0241060.0640.0330.07214.867.7116.78
O25B31.547350.0560.0600.08212.9413.9319.10
O25B41.070120.0530.0760.09312.3317.7521.72
O25BOverall 0.0560.0600.08313.0513.9619.19
  • a Precision experiments used human serum. Precision was evaluated on the basis of the results of 4 runs performed by 2 operators using a pool of high-potency serum samples that were diluted to 4 or 5 concentrations, as indicated.

  • b Theoretical titers at the original and log10 scale.

  • c CV, coefficient of variation.