TABLE 1

CD4 T cell peptide epitopes recognized by HLA-DR1 and HLA-DR4 transgenic micea

MHC-II restrictionHA proteinPeptide nameAmino acid sequenceAvg no. of spots/106 T cells
HLA-DR1pH1aa221221 SRYSKKFKPEIAIRP 235405
aa225225 KKFKPEIAIRPKVRD 239476
aa305305 TSLPFQNIHPITIGK 319578
aa437437 TYNAELLVLLENERT 451188
aa441441 ELLVLLENERTLDYH 455318
H3aa113113 CYPYDVPDYASLRSLVA 129732
aa119119 PDYASLRSLVASSGTLE 135965
aa315315 RITYGACPRYVKQNTLK 331127
aa321321 CPRYVKQNTLKLATGMR 3371,697
aa386386 TQAAINQINGKLNRLIG 402139
aa391391 NQINGKLNRLIGKTNEK 407166
H5aa157157 TSFFRNVVWLIKKNSTY 173807
aa259259 SNGNFIAPEYAYKIVKK 2751,484
aa301301 INSSMPFHNIHPLTIGE 317172
aa307307 FHNIHPLTIGECPKYVK 323311
aa433433 VWTYNAELLVLMENERT 449184
aa439439 ELLVLMENERTLDFHDS 455474
H7aa8181 PPQCDQFLEFSADLIIE 97223
aa106106 YPGKFVNEEALRQILRE 122803
aa181181 RKSPALIVWGIHHSVST 197909
aa241241 FHWLMLNPNDTVTFSFN 257275
aa266266 ASFLRGKSMGIQSGVQV 282473
aa271271 GKSMGIQSGVQVDANCE 287114
aa336336 PKGRGLFGAIAGFIENG 352200
aa341341 LFGAIAGFIENGWEGLI 357119
aa381381 QSAIDQITGKLNRLIEK 397339
aa386386 QITGKLNRLIEKTNQQF 402339
aa406406 DNEFNEVEKQIGNVINW 422111
aa431431 WSYNAELLVAMENQHTI 447506
aa436436 ELLVAMENQHTIDLADS 452476
HLA-DR4pH1aa269269 RYAFAMERNAGSGII 283240
H3aa3737 PNGTIVKTITNDQIEVT 53265
aa315315 RITYGACPRYVKQNTLK 331155
aa321321 CPRYVKQNTLKLATGMR 337685
H5aa6161 DGVKPLILRDCSVAGWL 77559
aa439439 ELLVLMENERTLDFHDS 45573
H7aa106106 YPGKFVNEEALRQILRE 122174
aa156156 AEMKWLLSNTDNAAFPQ 172304
aa181181 RKSPALIVWGIHHSVST 197269
aa511511 QIDPVKLSSGYKDVILW 527141
  • a Characteristics of major epitopes are indicated in boldface.