TABLE 1

CD4 T cell peptide epitopes recognized by HLA-DR1 and HLA-DR4 transgenic micea

MHC-II restrictionHA proteinPeptide nameAmino acid sequenceAvg no. of spots/106 T cells
HLA-DR1pH1 aa221 221 SRYSKKFKPEIAIRP 235 405
aa225 225 KKFKPEIAIRPKVRD 239 476
aa305 305 TSLPFQNIHPITIGK 319 578
aa437437 TYNAELLVLLENERT 451188
aa441441 ELLVLLENERTLDYH 455318
H3 aa113 113 CYPYDVPDYASLRSLVA 129 732
aa119 119 PDYASLRSLVASSGTLE 135 965
aa315315 RITYGACPRYVKQNTLK 331127
aa321 321 CPRYVKQNTLKLATGMR 337 1,697
aa386386 TQAAINQINGKLNRLIG 402139
aa391391 NQINGKLNRLIGKTNEK 407166
H5 aa157 157 TSFFRNVVWLIKKNSTY 173 807
aa259 259 SNGNFIAPEYAYKIVKK 275 1,484
aa301301 INSSMPFHNIHPLTIGE 317172
aa307307 FHNIHPLTIGECPKYVK 323311
aa433433 VWTYNAELLVLMENERT 449184
aa439 439 ELLVLMENERTLDFHDS 455 474
H7aa8181 PPQCDQFLEFSADLIIE 97223
aa106 106 YPGKFVNEEALRQILRE 122 803
aa181 181 RKSPALIVWGIHHSVST 197 909
aa241241 FHWLMLNPNDTVTFSFN 257275
aa266 266 ASFLRGKSMGIQSGVQV 282 473
aa271271 GKSMGIQSGVQVDANCE 287114
aa336336 PKGRGLFGAIAGFIENG 352200
aa341341 LFGAIAGFIENGWEGLI 357119
aa381381 QSAIDQITGKLNRLIEK 397339
aa386386 QITGKLNRLIEKTNQQF 402339
aa406406 DNEFNEVEKQIGNVINW 422111
aa431 431 WSYNAELLVAMENQHTI 447 506
aa436 436 ELLVAMENQHTIDLADS 452 476
HLA-DR4pH1 aa269 269 RYAFAMERNAGSGII 283 240
H3 aa37 37 PNGTIVKTITNDQIEVT 53 265
aa315315 RITYGACPRYVKQNTLK 331155
aa321 321 CPRYVKQNTLKLATGMR 337 685
H5 aa61 61 DGVKPLILRDCSVAGWL 77 559
aa439439 ELLVLMENERTLDFHDS 45573
H7aa106106 YPGKFVNEEALRQILRE 122174
aa156 156 AEMKWLLSNTDNAAFPQ 172 304
aa181 181 RKSPALIVWGIHHSVST 197 269
aa511511 QIDPVKLSSGYKDVILW 527141
  • a Characteristics of major epitopes are indicated in boldface.