TABLE 1

Relative protection of mice against Borrelia infection through immunization with dimeric and monomeric recombinant OspC constructs

AntigenChallengeNo. of animalsDosage (μg)No. of immunizationsNo. infected/total no.% protection
Summary, experiments with B31 immunization and challenge
    Alum onlyB31270427/270
    Alum + OspC-DB31B31630.1–10042/6097
    Alum + OspC-MB31B31450.1–100432/3918
    MPL onlyB313033/30
    MPL + OspC-DB31B316130/6100
    MPL + OspC-MB31B316133/650
Experiment 1, antigen dosage effects
    Alum onlyB316046/60
    Alum + OspC-DB31B31610040/6100
    Alum + OspC-DB31B3163040/6100
    Alum + OspC-DB31B3161040/6100
    Alum + OspC-M31B31610045/617
    Alum + OspC-MB31B3163045/617
    Alum + OspC-MB31B3161044/633
Experiment 2, antigen dosage effects
    Alum onlyB316046/60
    Alum + OspC-DB31B3161041/683
    Alum + OspC-DB31B316140/6100
    Alum + OspC-DB31B3160.141/683
    Alum + OspC-MB31B31610044/633
    Alum + OspC-MB31B3131042/333
    Alum + OspC-MB31B313143/30
    Alum + OspC-MB31B3130.143/30
Experiment 3, no. of immunizations
    Alum onlyB313043/30
    Alum + OspC-DB31B316140/6100
    Alum + OspC-DB31B316131/683
    Alum + OspC-DB31B316122/667
    Alum + OspC-MB31B316146/60
Experiment 4, no. of immunizations
    Alum onlyB313043/30
    Alum + OspC-DB31B316140/6100
    Alum + OspC-DB31B316131/683
    Alum + OspC-DB31B316124/633
Experiment 5, cross-strain challenge
    Alum onlyB313043/30
    Alum + OspC-DB31B316140/6100
    Alum onlyN406046/60
    Alum + OspC-DB31N406146/60
    Alum only2976046/60
    Alum + OspC-DB312976146/60
Experiment 6, different lots of recombinant proteins
    Alum onlyB313143/30
    Alum + OspC-DB31 (lot 1)B313140/3100
    Alum + OspC-DB31 (lot 2)B313140/3100
    Alum + OspC-DB31 (lot 3)B313140/3100
    Alum + OspC-MB31 (lot 2)B313143/30
    Alum + OspC-MB31 (lot 3)B313141/367
Experiment 7, OspC from different strains
    Alum only2973043/30
    Alum + OspC-D2972976140/6100
    Alum + OspC-M2972976145/617
    Alum onlyN403043/30
    Alum + OspC-DN40N406140/6100
    Alum + OspC-MN40N406140/6100
Experiment 8, Cross-strain challenge, MPL adjuvant, and reduced dimer immunization
    MPL onlyB313133/30
    MPL + OspC-MB31B316133/650
    MPL + OspC-DB31B316130/6100
    MPL + OspC-DB31B316122/667
    MPL + OspC-DN40B313133/30
    None2973133/30
    MPL + OspC-DN402973132/333
    MPL onlyN403133/30
    MPL + OspC-DN40N403130/3100