TABLE 4

Fecal shedding of S. Senftenberg from chickens inoculated with JOL1587

Groupa1 dayb3 days7 days9 days14 days21 days
No. of positive samples/total no. of samplescRecovery (log10 CFU/g) (mean ± SEM)dNo. of positive samples/total no. of samplesRecovery (log10 CFU/g) (mean ± SEM)No. of positive samples/total no. of samplesRecovery (log10 CFU/g) (mean ± SEM)No. of positive samples/total no. of samplesRecovery (log10 CFU/g) (mean ± SEM)No. of positive samples/total no. of samplesRecovery (log10 CFU/g) (mean ± SEM)No. of positive samples/total no. of samplesRecovery (log10 CFU/g) (mean ± SEM)
A0/50 ± 00/50 ± 00/50 ± 00/50 ± 00/50 ± 00/50 ± 0
B1/5e3.8 ± 1.0f1/5e2.0 ± 1.0f2/5e2.3 ± 0.2f0/50 ± 00/50 ± 00/50 ± 0
C1/5e1.9 ± 1.0f5/5e4.2 ± 1.2f2/5e2.1 ± 1.5f0/50 ± 00/50 ± 00/50 ± 0
  • a Group A, PBS-treated control group; group B, chickens orally inoculated with JOL1587 (Δlon ΔcpxR); group C, chickens intramuscularly inoculated with JOL1587 (Δlon ΔcpxR).

  • b Days postinoculation.

  • c Number of positive samples per total number of samples observed with the enrichment culture method.

  • d Bacterial recovery was determined by direct and enrichment culturing of fecal samples from birds at 1, 3, 7, 9, 14, and 21 days postinoculation.

  • e Significantly greater number of S. Senftenberg-positive chickens in the immunized group, compared to the control group, postinoculation (P ≤ 0.05).

  • f Significantly greater bacterial recovery of S. Senftenberg from feces from chickens in the immunized group, compared to the control group, postinoculation (P ≤ 0.05).