TABLE 8

B. burgdorferi antigens recognized by immunized mouse seruma

Band size (kDa) or parameterNo. of samples positive/total
PrechallengePostchallenge with B31-A3-infected tick
PBSΔp66p66cpPBSΔp66p66cp
180/40/50/52/50/50/5
230/41/50/53/51/53/5
300/45/55/53/55/55/5
310/42/55/50/54/53/5
390/42/50/52/53/52/5
410/44/55/52/55/55/5
450/41/53/50/54/53/5
580/40/50/51/50/50/5
660/41/53/50/54/53/5
930/40/50/50/50/50/5
All bands0/4016/50b21/50b13/5026/50c24/50c
No. of mice seropositive/totald0/41/5e3/5e2/54/5e3/5e
  • a Prechallenge mice were immunized four times at 2-week intervals. Two weeks after the last immunization, serum samples were collected. Postchallenge mice were immunized four times and then challenged 4 weeks after the last immunization with B31-A3-infected ticks. Serum samples were collected 2 weeks after the challenge. Serum samples were used in a Gold Standard Diagnostics IgG line blot assay to detect B. burgdorferi proteins. Band intensity equal or greater than the cutoff control intensity was interpreted as positive.

  • b P < 0.0001.

  • c P < 0.05.

  • d A sample was considered B. burgdorferi IgG seropositive if five or more bands were present. The molecular masses of the test proteins used were 18, 23 (OspC), 30, 31 (OspA), 39 (BmpA), 41 (Fla), 45, 58 (OppA-2), 66, and 93 kDa. The number of bands to which each serum sample reacted and the seropositivity (defined as five or more bands reactive) of Δp66- or p66cp-immunized mice were compared to those of PBS controls with Fisher's exact test in GraphPad Prism.

  • e No statistically significant difference.