TABLE 1

NHP immunization and challenge timeline

Group (n)Induction of preexisting antiadenovirus immunity (day 0)Prime vaccination (day 30)Boost with rYFV (day 42)Aerosol challenge (day 85)
Immunized (4)5 × 1010 v.p./250 μl Ad5-empty, i.m. route1 × 1011 v.p./500 μl rAd5-YFV, i.n. route (250 μl per nostril)50 μg rYFV mixed with alhydrogel (250 μl), i.m. routeWT CO92 (Dp, 1.32 × 107–8.08 × 107 CFU)
Control (4)Saline (250 μl), i.m. routeSaline (500 μl), i.n. route (250 μl per nostril)Saline (250 μl), i.m. route