TABLE 4

Protection against different strains of LD borreliae inoculated via syringea

ImmunogenChallenge strainNo. of tissue cultures positive/totalNo. of mice protected/total
BladderHeartTibioSkinEar
PBS-NMSB. burgdorferi N40 D10/E95/55/55/55/55/50/5
Δp66 bacteriaB. burgdorferi N40 D10/E90/5b0/50/50/50/55/5c
p66cp bacteriaB. burgdorferi N40 D10/E90/5b0/50/50/50/55/5c
PBS-NMSB. garinii PBr1/52/50/55/53/50/5
Δp66 bacteriaB. garinii PBr0/50/50/52/50/53/5d
p66cp bacteriaB. garinii PBr0/50/50/55/55/50/5d
  • a All mice were immunized four times at 2-week intervals. Mice were challenged with 1 × 105 N40 D10/E9 bacteria only 1 week after the last immunization and euthanized 4 weeks after the challenge. Those challenged with PBr (1 × 105 bacteria) were challenged at 4 weeks after the last immunization and euthanized 2 weeks after the challenge. Tibio refers to tibiotarsal joint, skin refers to the area of skin between the shoulder blades at which the mice were injected with bacteria, and ear refers to a sample of the margin of the ear distal to the head. In each group, Δp66- or p66cp-immunized mice were compared to the PBS-NMS-treated controls with Fisher's exact test in GraphPad Prism.

  • b P < 0.0001.

  • c P < 0.01.

  • d No statistically significant difference.