Summary of the properties of the established MAbs analyzed by different serological assays

MAb cloneImmunogenIsotypeIndirect IgG ELISAaIFA resultbYFV-E Western blot result (E. coli)cDomain specificitydFRNT50e
YF-17DDENV2JEVYF-17DBaringo 1Baringo 2DENV2JEVYF-17DBaringo 2
5H2E proteinIgG2bκ+++++++++DoIIR1<10<10
4A1E proteinIgG1κ+++++ND<10<10
4C9E proteinIgG1κ+++++++++DoIR1<10<10
4H10E proteinIgG2bκ+++++++++DoIR1<10<10
3B617D virusIgG2bκ+++++ND<10<10
5B617D virusIgG1κ+++++DoIIR1<10<10
3F417D virusIgG2aκ++++++++DoIR1<10<10
8H317D virusIgG1κ++++++++ND<10<10
  • a The reactivity of MAbs with selected flaviviruses was determined by indirect IgG ELISA. +, positive; −, negative.

  • b IFA fluorescence intensity was scored as follows: −, no fluorescence detectable; +, intermediate reactivity; ++, high fluorescence intensity.

  • c The reactivity of MAbs with YFV-E protein was determined by Western blot analysis. −, no detectable signal; +, weak positive signal; ++, strong positive signal.

  • d ND, not determined.

  • e FRNT50, neutralization titer. The neutralization titer was determined as the reciprocal of the MAb dilution that reduced the number of foci by 50% or more in wells with MAb compared to negative-control wells.