TABLE 1

Recovery yields of the different NS5 purification stepsa

VariableValueb (mg/liter)Yield (%)
After affinity chromatographyc20.23
After dialysisd19.6897.28
After SECe7.6437.80
NS5f0.5
NS5g3.0
  • a The recovery yields refer to 1 liter of IPTG-induced culture.

  • b Value represents the NS5 yield relative to 1 liter of IPTG-induced culture, determined by the BCA method (Pierce BCA protein assay kit, Thermo Scientific).

  • c All NS5-containing samples obtained after affinity chromatography.

  • d Sample obtained after dialysis of the pooled fractions recovered from affinity chromatography.

  • e Sample obtained after the size exclusion chromatography (SEC) step.

  • f Previously reported yield for recombinant DENV1 GST-NS5 (51).

  • g Previously reported yield for recombinant DENV3 NS5 polymerase domain (52).