Effects of different reagents on catalase A1 activity

Reagent (final concn)Residual activity (%)a
Potassium cyanide (10 mM)0
Sodium azide (10 mM)0
3-AT (4 mM)38
Ethanol-chloroform (25%–15%)71
Cu2+ (10 mM)52
Hg2+ (10 mM)14
SDS (4%)97
2-ME (30 mM)14
  • a Residual activity was determined spectrophotometrically after incubation of the purified enzyme for 1 h in the presence of the different reagents tested.