TABLE 1

Protection of mice against challenge with B. abortus strain 2308 after immunization with DNA vaccine formulations

Vaccine (dose)Mice (n = 5) challenged intraperitoneally with 104 CFU of B. abortus strain 2308
Log10 CFU of B. abortus 2308 in spleen (mean ± SD)Log10 units of protection
PBS6.02 ± 0.520
pcDNA3.1 (50 μg)6.16 ± 0.480
pcDNA-SOD (50 μg)3.86 ± 0.352.16a,b
pcDNA3.1 (50 μg) and BPPcysMPEG (5 μg)6.12 ± 0.390
pcDNA-SOD (50 μg) and BPPcysMPEG (5 μg)2.73 ± 0.223.29a,b
B. abortus strain RB512.47 ± 0.303.55a
  • a α = 0.05 compared to the negative-control group receiving PBS.

  • b The difference between pcDNA-SOD and pcDNA-SOD/BPPcysMPEG is statistically significant, with values compared using Sidak's multiple-comparisons test (α = 0.05).