Criteria for scoring clinical observations of micea

ScoreClinical symptom criteria
0No clinical symptoms
1Rough coat, rash, nervous behavior
2Spiky hair, pock formation (no suppuration)
3Pock formation (suppuration), loss of hair, hunched over, inactive, slow moving
4Paralysis, moribund, abnormal behavior (stumbling, etc.)
  • a The evaluation of clinical symptoms was expressed as a score for each mouse. Each group's total clinical score for every observation day was expressed as the sum of each mouse score.