TABLE 2

Comparative ELISA assessment of OppA2 peptides as serodiagnostic markers for Lyme disease

Peptide (amino acids comprising the peptide)Reaction resultAntibody panel results (% [n]) of samples from:
All Lyme disease patientsd (n = 104)Controls
Healthy donors (n = 44)RAe patients (n = 30)Syphilis patients (n = 26)
OppA (191-225)Positivea44.2 (46)4.5 (2)0 (0)11.5 (3)
Equivocalb17.3 (18)0 (0)0 (0)7.7 (2)
Negativec38.5 (40)95.5 (42)100 (30)80.8 (21)
OppA (381-400)Positive40.4 (42)4.5 (2)0 (0)7.7 (2)
Equivocal16.3 (17)2.3 (1)0 (0)3.8 (1)
Negative43.3 (45)93.2 (41)100 (30)88.5 (23)
  • a At least 3 SD from the mean absorbance of healthy donor samples.

  • b Between 2 SD and 3 SD from the mean absorbance of healthy donor samples.

  • c Less than 2 SD from the mean absorbance of healthy donor samples.

  • d All Lyme disease patients presented with one or more erythema migrans lesion(s) and resided in geographical regions where Lyme disease is endemic.

  • e RA, rheumatoid arthritis.