TABLE 2

Characterization of pertactin-deficient isolates by sequencing analyses

Mutation typeaPertactin mutation, nucleotide no.Pertactin MLSTb allelePredicted pertactin proteincState (no. of isolates)Year (no. of isolates)PFGEd (no. of isolates)
IIS481, 246eprn2Stop at aa 87fWA (2)2011 (1) 2012 (1)CDC 253 (2)
IIIS481, 1613eprn2Stop at aa 543WA (8)2012 (8)CDC 237 (4) CDC 013 (2) CDC 010 (1) CDC 002 (1)
IIIIS481, 2735eprn2Stop at aa 913WA (8)2012 (7)CDC 322 (6) CDC 002 (1)
2011 (1)CDC 046 (1)
NY (1)2012 (1)CDC 046 (1)
IVStop, 760prn2Stop at aa 254NY (4)2011 (1) 2012 (3)CDC 242 (4)
VStop, 1273prn2Stop at aa 425VT (1)2011 (1)CDC 002 (1)
NY (1)2012 (1)CDC 002 (1)
VIInsert G, 1185prn2Frameshift at aa 395MN (2)2012 (2)CDC 002 (2)
VIIDeletion, 26–109prn1Deletion of 28 aaCA (1)2010 (1)CDC 268 (1)
WA (2)2012 (2)CDC 260 (2)
VIIIDeletion, −2090 to 478prn2Deletion of 2.6 kbWA (2)2012 (2)CDC 013 (1) CDC 046 (1)
IXInversion, −74prn2Inversion of ∼22 kb in promoterWA (16)2011 (2)CDC 010 (2)
2012 (14)CDC 010 (12) CDC 002 (2)
XWild typeprn2Full-length 915 aaCA (1)2010 (1)CDC 013 (1)
  • a At least one isolate of each mutation type has been confirmed by Western blot analysis to lack protein expression.

  • b MLST, multilocus sequence typing.

  • c prn lies in the region between nt 1,098,091 and 1,100,823 of the Tohama I complete genome (GenBank accession no. NC_002929.2).

  • d PFGE, pulsed-field gel electrophoresis.

  • e Shows forward IS481 insertion as example, according to GenBank accession no. M22031.

  • f Stop, stop codon; aa, amino acid.