TABLE 1

Binding characteristics and Ab63/Ab52 competitor potency of blood serum samples from tularemia patients

Patient no.Serum no.Titers by binding characteristics:Serum dilution at 50% inhibition of MAb binding to LPSa
Microagglutination of LVSELISA binding to LPS (IgM + IgG + IgA)ELISA binding to OAgC (IgM + IgG + IgA)Ab63Ab52Normalized Ab63/Ab52c
P1S1≥8,192182,250546,7507,6591,05411.9
S2≥8,1921,640,250546,75013,2741,44515.0
P2S3≥8,192182,250182,2504,1398298.2
P3S451220,25020,25063119.5
S551220,25020,250105≤8b≥21.5
P4S651220,25060,75084112910.7
P5S71282,2506,75055≤2b≥45.1
S81282,2506,75046≤1b≥74.6
P6S92,048182,25060,7508822346.1
S101,024182,25060,7505953572.7
P7S111282,2506,75066147.9
S122,048182,25060,7502302614.6
P8S131,02420,2506,7504011350.0
P9S141,02460,75060,7502452871.4
S151,02460,75060,7502242361.6
S161,02460,75020,2501692761.0
P10S172566,7506,75098237.0
P11S185126,7506,75049581.4
  • a Serum dilutions required for 50% inhibition were calculated from the linear parts of the graphs in Fig. 1.

  • b Extrapolated by drawing a parallel line to the Ab63 graph from the Ab52 point at the lowest serum dilution.

  • c The Ab63/Ab52 ratios were normalized to the lowest ratio (for S16) by dividing all other ratios by the S16 ratio, making the S16 ratio 1.0.