Table 1

Comparative bioinformatic analysis of the 4 Mycobacterium avium subsp. paratuberculosis antigens and mycobacterial homologs of pathogenic and diagnostic value

M. avium subsp. paratuberculosis genePfam domainProtein size (kDa)Similar epitope (% similarity)No. of common epitopes for M. avium subsp. paratuberculosis vs M. bovis/no. predicteda
M. avium 104M. bovis AF2122/97M. tuberculosis H37RvM. leprae TN
1569FAP36Mav2859 (95)Mb1891 (74)Rv1860 (74)ML2055 (74)2/12
0471Shisa28Mav0566 (99)Mb3618c (94)Rv3587c (94)ML1918 (90)2/7
1981cDUF16427Mav2210 (99)Mb2254c (90)Rv2229c (90)ML1638 (80)1/7
0196cSeptum_form46Mav0192 (98)Mb3865 (81)Rv3835 (81)ML0081 (83)1/15
  • a Standard protein BLAST was used to search for mycobacterial homologs, which were subsequently analyzed for percent similarity using FASTA sequence comparison (see http://fasta.bioch.virginia.edu). Bioinformatic prediction of common B-cell epitopes was performed for M. avium subsp. paratuberculosis homologs in M. bovis using Bepipred Linear Epitope Prediction (IEDB Analysis Resource) (see http://tools.immuneepitope.org). Data are presented as the total number of common epitopes over the total number of M. avium subsp. paratuberculosis epitopes predicted; only epitopes of ≥4 amino acids in length were considered.