Table 1

Comparative bioinformatic analysis of the 4 Mycobacterium avium subsp. paratuberculosis antigens and mycobacterial homologs of pathogenic and diagnostic value

M. avium subsp. paratuberculosis genePfam domainProtein size (kDa)Similar epitope (% similarity)No. of common epitopes for M. avium subsp. paratuberculosis vs M. bovis/no. predicteda
M. avium 104M. bovis AF2122/97M. tuberculosis H37RvM. leprae TN
1569FAP36Mav2859 (95)Mb1891 (74)Rv1860 (74)ML2055 (74)2/12
0471Shisa28Mav0566 (99)Mb3618c (94)Rv3587c (94)ML1918 (90)2/7
1981cDUF16427Mav2210 (99)Mb2254c (90)Rv2229c (90)ML1638 (80)1/7
0196cSeptum_form46Mav0192 (98)Mb3865 (81)Rv3835 (81)ML0081 (83)1/15
  • a Standard protein BLAST was used to search for mycobacterial homologs, which were subsequently analyzed for percent similarity using FASTA sequence comparison (see Bioinformatic prediction of common B-cell epitopes was performed for M. avium subsp. paratuberculosis homologs in M. bovis using Bepipred Linear Epitope Prediction (IEDB Analysis Resource) (see Data are presented as the total number of common epitopes over the total number of M. avium subsp. paratuberculosis epitopes predicted; only epitopes of ≥4 amino acids in length were considered.