Table 2

Significant effects of exercise stress on cytokine levels measured by three different assays

Assay type and cytokine testedaData for each groupb
ControlCFSCFS onlyCFS+FM
NormalExerciseNormalExerciseNormalExerciseNormalExercise
ELISPOT assay171596
    IL-1βc74.4 ± 50.2109.9 ± 62.6f28.4 ± 11.926.4 ± 8.037.3 ± 18.329.0 ± 10.715.0 ± 8.922.5 ± 12.0
    IL-4c6.9 ± 1.58.4 ± 2.611.6 ± 2.47.7 ± 1.8f12.5 ± 2.97.4 ± 2.4f10.2 ± 4.18.2 ± 2.7
    IL-6c47.2 ± 11.469.2 ± 16.274.7 ± 21.751.0 ± 20.4f50.9 ± 18.28.1 ± 3.2f110.0 ± 43.0115.2 ± 38.0g
    ST IL-6c96.8 ± 14.1117.2 ± 16.3118.5 ± 16.795.1 ± 16.7f108.4 ± 11.867.9 ± 16.9f134.0 ± 36.6135.7 ± 25.5
    IL-8c36.2 ± 8.554.9 ± 13.657.8 ± 10.124.6 ± 5.4f58.9 ± 14.025.8 ± 5.956.1 ± 13.922.8 ± 10.2f
    ST IL-8c50.1 ± 10.062.9 ± 13.572.2 ± 7.832.0 ± 7.5f60.6 ± 11.131.4 ± 10.0f89.0 ± 6.433.0 ± 11.1f
    TNF-αc145.1 ± 28.0182.6 ± 30.3f130.0 ± 33.4151.3 ± 22.5f157.6 ± 46.5148.7 ± 27.388.6 ± 40.7155.0 ± 38.6f
Luminex assay171596
    IL-1βd0.09 ± 0.040.11 ± 0.050.33 ± 0.110.34 ± 0.120.16 ± 0.070.16 ± 0.070.57 ± 0.22h0.60 ± 0.25
    IL-4d0.18 ± 0.080.10 ± 0.03f0.07 ± 0.030.06 ± 0.020.02 ± 0.020.06 ± 0.030.15 ± 0.070.08 ± 0.05
    IL-10d2.22 ± 0.391.40 ± 0.25f2.37 ± 0.342.24 ± 0.342.02 ± 0.432.07 ± 0.492.89 ± 0.542.49 ± 0.37
qRT-PCR assay (n)e1817107
  • a ST, stimulant-induced data.

  • b The statistical tests were conducted with log-transformed data. Values in bold type indicate significant cases.

  • c No. of cytokine-producing cells (mean ± SE) (raw data).

  • d Cytokine level (mean ± SE) (pg/ml) (raw data).

  • e There were no significant cases for the qRT-PCR assay.

  • f Significant within-group difference from normal night.

  • g Significant difference between CFS only and CFS+FM.

  • h Significant difference between control subjects and patients with CFS+FM.