Table 2

Optimized conditions for anti-TcdA and anti-TcdB NAb assays

NAbCell lineCell seeding density (no. of cells/well)Toxin concn (ng/ml)Mean preincubation time (min) ± SEMMean cell intoxication time (h) ± SEM
Anti-TcdAVero750475 ± 1546 ± 2
Anti-TcdBVero7500.0475 ± 1546 ± 2