Table 7

Relationship among a wide selection of hemagglutinin proteins of H5N1 and H5N2 avian influenza virus strains, including four used in this studya

StrainAmino acid residuesMatchNonmatch% Matchb
A/Hanoi/30408/2005 (H5N1)567
A/blue-winged teal/Saskatchewan/22542/2007 (H5N2)5645036489
A/Cambodia/R0405050/2007 (H5N1)5655551398
A/Cambodia/V0606311/2011 (H5N1)5685491997
A/Cambodia/W0112303/2012 (H5N1)5685531597
A/cat/Germany/R606/06 (H5N1)5685472196
A/chicken/Cambodia/7/2004 (H5N1)5535491999
A/chicken/Egypt/1/2008 (H5N1)5685373195
A/chicken/Hebei/326/2005 (H5N1)5485155394
A/Ck/HK/61.9/02 (H5N1)5485353398
A/Chicken/Hong Kong/220/97 (H5N1)5685422695
A/chicken/Nigeria/08VIR4337-343/2008 (H5N1)5425175195
A/chicken/Indonesia/D10015/2010 (H5N1)5685412795
A/chicken/Mexico/31381-7/1994 (H5N2)5644927587
A/chicken/Nigeria/1047-30/2006 (H5N1)5685472196
A/chicken/Pennsylvania/1370/1983 (H5N2)5474709786
A/chicken/Rostov/22-1/2007 (H5N1)5685442496
A/chicken/Scotland/1959 (H5N1)5645135491
A/chicken/Vietnam/14/2005 (H5N1)5675402795
A-chicken-Vietnam-NCVD-swab276/20095504957690
A/chicken/Xinjiang/28/2006 (H5N1)5525254395
A/clouded leopard/Chonburi/AI-1216A/2004 (H5N1)564559999
A/common coot/Switzerland/V544/2006 (H5N1)5685452396
A/common magpie/Hong Kong/5052/2007 (H5N1)5555274095
A/domestic duck/Bucharest/RO-AI-482/2006 (H5N1)5465244496
A/duck/Badung-Bali/05/2005 (H5N1)5295056395
A/duck/PA/454069/2005 (H5N1)5645026589
A/duck/Cambodia/D1KP/2006 (H5N1)568559998
A/duck/Hunan/795/2002 (H5N1)5655482097
A/duck/Laos/3295/2006 (H5N1)5675442496
A/duck/Minnesota/1525/1981 (H5N1)5645046389
A/Egypt/2321-NAMRU3/2007 (H5N1)5685442496
A/Egypt/2514-NAMRU3/2008 (H5N1)5495274196
A/equine/Egypt/av1/2009 (H5N1)5675363295
A/goose/Crimea/615/05 (H5N1)5685472196
A/domestic goose/Germany/R1400/07 (H5N1)5685422695
A/Goose/Guangdong/1/96 (H5N1)5685442496
A/goose/Guiyang/337/2006 (H5N1)5565274195
A/goose/Hungary/14756/2006 (H5N1)5685462296
A/Guangdong/2/2006 (H5N1)5675422596
A/Hong Kong/156/97 (H5N1)5685422695
A/Hong Kong/6841/2010 (H5N1)5284887992
A/Indonesia/CDC1032/2007 (H5N1)5525274195
A/mallard/British Columbia/18311/2006 (H5N2)5645036489
A/mallard/Ontario/26078/2007 (H5N1)5645016689
A/mallard/Quebec/11200/2006 (H5N2)5514937489
A/quail/Vietnam/177/2004 (H5N1)5655571199
A/swine/Fujian/2001 (H5N1)5685501897
A/swine/Guangxi/wz/2004 (H5N1)5685531597
A/tree sparrow/Indonesia/D10013/2010 (H5N1)5685363294
A/Canada goose/Wyoming/473197-10/2006 (H5N2)5645006489
A/turkey/Ontario/6213/1966 (H5N9)5644996888
A/turkey/Ontario/7732/1966 (H5N9)5654917687
A/turkey/Turkey/1/2005 (H5N1)5685452396
  • a A total of 54 hemagglutinin sequences obtained from sources indicated below were analyzed for similarities with the hemagglutinin sequence used for constructing the pCAG-HA DNA vaccine, A/Hanoi/30408/2005 (H5N1), as the reference sequence. Global sequence alignment analysis was performed using the BLOSUM 62 scoring matrix (Clone Manager Professional v9.2, Scientific & Educational Software, Cary, NC). Sources of hemagglutinin sequences: http://www.fludb.org/brc/home.do?decorator=influenza and http://www.ncbi.nlm.nih.gov/genomes/FLU/.

  • b The % match is the proportion of matching amino acids compared to the total number of amino acid residues.