Table 2

Amino acid mutations between JXA1 and the derivative viruses

ORFPositionaMutation at indicated no. of passagesb
580100110120130140150160170
ORF1a
    nsp1β187DGGGGGGGGG
239RKKKKKKKKK
242FLLLLLLLLL
378SSSRRRRRRR
    nsp2572LLLSSSSSSS
636TAAAAAAAAA
679VEEEEEEEEE
686EDDDDDDDDD
780KKKKQEEEEE
782ATTTTTTTTT
902GGGGGGGGEE
928AVVVVVVVVV
951GRRRRRRRRR
957EGGGGGGGGG
1062RMMMMMMMMM
1128VAAAAAAAAA
1155IMMMMMMMMM
1179GDDDDDDDDD
1392SLLLLLLLLL
1561RCCCCCCCCC
    nsp31629EDDDDDDDDD
1723IIITTTTTTT
    nsp7α2331NSSSSSSSSS
ORF1b
    nsp9138KEEEEEEEEE
365IMMMMMMMMM
423EGGGGGGGGG
    nsp10847RHHHHHHHHH
1036ATTTTTTTTT
1038PHHHHHHHHH
1040EDDDDDDDDD
1059VLLLLLLLLL
1136AVVAAAAAAA
    nsp111181MVVVVVVVVV
ORF210LFFFFFFFFF
23NSSSSSSSSS
50YFFFFFFFFF
118IVVVVVVVVV
168MTTMMMMMMM
204HHHNNNNNNN
251FSSSSSSSSS
    E3SSSAAAAAAA
9DHHHHHHHHH
48LFFFFFFFFF
ORF312FLLLLLLLLL
79HNNNNYYYYY
85NNNNNNNSSS
165FFFFFFFFYY
196VVVVVVVVII
216SSSSSSSSSL
225TAAAAAAAAA
248FVVVVVVVVV
ORF414CCCCCCCCYY
43DGGGGGGGGG
66ISSSSSSSSS
124IIVVVVVVVV
132STTTTTTTTT
172LVVFFFFFFF
173PAAAAAAAAA
174SIIIIIIIII
ORF5a36QQQRRRRRRR
49NDDDDDDDDD
ORF533NNNDDDDDDD
59KNNNNNNNNN
164GRRRRRRRRR
ORF711KKKKKKKNNN
  • a The cleavage positions were determined based on a previous report (9).

  • b Mutations correlated with the attenuation are shown in lightface characters without underlining; mutations responsible for the overattenuation are shown in boldface characters without underlining; mutations might also contribute to the overattenuation are shown in lightface characters with underlining; two reversion mutations (aa 1136 in nsp10 and aa 168 in ORF2) that are probably not correlated with the attenuation or overattenuation of JXA1 are shown in boldface characters with underlining.