Table 2

Cytokine analysis from cells isolated from rBmAsnRS-treated versus untreated T-cell transfer colitis mice

Cytokine analysisBaseline and stimulated cytokine levela
CellsCD3+LPSCPG
Spleen
    IFN-γUntreated0.045 ± 0.0172.251 ± 0.030.657 ± 0.0660.614 ± 0.101
rBmAsnRS0.014 ± 0.012.086 ± 0.1790.386 ± 0.043*0.304 ± 0.064
    IL-17UntreatedBD2.102 ± 0.0210.72 ± 0.12BD
rBmAsnRSBD1.556 ± 0.270.641 ± 0.053BD
    IL-4UntreatedBD0.664 ± 0.0320.322 ± 0.010.343 ± 0.041
rBmAsnRSBD0.707 ± 0.0220.387 ± 0.0410.473 ± 0.066
    IL-10Untreated0.242 ± 0.060.271 ± 0.0391.951 ± 0.051.562 ± 0.128
rBmAsnRS0.122 ± 0.0130.898 ± 0.031*2.966 ± 0.03*3.019 ± 0.025*
Mesenteric lymph nodes
    IFN-γUntreated0.399 ± 0.013.009 ± 0.0830.706 ± 0.0971.836 ± 0.079
rBmAsnRS0.16 ± 0.0762.698 ± 0.0650.357 ± 0.0371.749 ± 0.132
    IL-17UntreatedBD5.549 ± 0.1641.170 ± 0.030.953 ± 0.122
rBmAsnRS0.087 ± 0.01*4.434 ± 0.144*0.256 ± 0.041*0.246 ± 0.052*
    IL-4UntreatedBD0.573 ± 0.028BD0.173 ± 0.048
rBmAsnRSBD0.818 ± 0.042*0.174 ± 0.01*0.518 ± 0.045*
    IL-10UntreatedBD1.394 ± 0.027BD0.539 ± 0.011
rBmAsnRS0.135 ± 0.0622.271 ± 0.033*2.23 ± 0.07*1.252 ± 0.018*
Lamina propria lymphocytes
    IFN-γUntreated0.072 ± 0.0181.158 ± 0.042BDBD
rBmAsnRS0.039 ± 0.010.78 ± 0.013*BDBD
    IL-17UntreatedBD1.842 ± 0.0360.74 ± 0.030.62 ± 0.12
rBmAsnRSBD1.545 ± 0.0490.618 ± 0.030.42 ± 0.16
    IL-4UntreatedBD0.154 ± 0.012BDBD
rBmAsnRSBD0.376 ± 0.01*BDBD
    IL-10UntreatedBD0.228 ± 0.05BDBD
rBmAsnRSBD0.367 ± 0.03BD0.129 ± 0.03*
  • a *, statistically significant values (P < 0.05) using Student's t test. BD signifies below detectable limits of the assay.