Table 1

Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaSource or reference
Strains
    Y. pestis
        CO92 BEIFully virulent WT strainBEIb
        Δcaf1Deletion mutant of the caf1 gene (F1)39
    E. coli BL21(DE3)F ompT gal dcm lon hsdSB(rB mB) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])Novagen
Plasmids
    pET-30a(+)T7 promoter-based prokaryotic expression vector; KmrNovagen
    pET-20b(+)T7 promoter-based prokaryotic expression vector; AprNovagen
    pET-30/ailail gene of Y. pestis cloned at BglII/SalI sites of pET-30a(+) vector for purification of Ail protein; KmrThis study
    pET-30/ompAompA gene of Y. pestis cloned at NdeI/XhoI sites of pET-30a(+) vector for purification of OmpA protein; KmrThis study
    pET-20/caf1caf1 gene of Y. pestis cloned at NdeI/XhoI sites of pET-20b(+) vector for purification of F1 protein; AprThis study
    pET-20/plapla gene of Y. pestis cloned at NdeI/XhoI sites of pET-20b(+) vector for purification of Pla protein; Apr38
  • a Apr, ampicillin resistance; Kmr, kanamycin resistance.

  • b BEI, The Biodefense and Emerging Infections Research Resources Repository, Manassas, VA.