Table 2

qRT-PCR analysis of 26 selected hypothetical unknown ORFs

ORFCT value for genomic DNAaCT value for cDNALevel of gene expressionb
100 pg10 pg1 pg500 fg100 fg50 fg10 fg
ML002324.626.938.937.137.938.6NA37.4500 fg
ML007025.230.733.633.53434.235.433.61 pg
ML012123.426.931.832.933.536.231.8500 fg
ML01413637.639.240.642.7NA36.51 pg
ML018823.929.135.436.436.8NA34.61 pg
ML021723.828.433.936.937.140.4321 pg
ML044821.725.229.133.334.231.4500 fg-1 pg
ML052722.425.429.230.232.433.228.9>1 pg
ML057321.624.728.833.434.8344132500 fg-1 pg
ML057423.12730.5313233.230.91 pg
ML058822.82630.531.832.734.830.11 pg
ML06142529.132.43637.5321 pg
ML075523.226.73131.833.135.431.7500 fg-1 pg
ML092023.827.431.132.53531.5500 fg-1 pg
ML09532729.533.835.139.234.1500 fg-1 pg
ML0959242932.533.436.232.61 pg
ML101022.425.228.730.232.232.928.51 pg
ML138422.725.230.43134.835.230.71 pg
ML157531.134.437.838.941.437.91 pg
ML194923.727.131.432.934.132500 fg-1 pg
ML204425.73033.734.73739.133.91 pg
ML230724.627.231.833.234.835.231.51 pg
ML23132630.534.335.838.732.31 pg
ML263022.826.530.931.632.9NA31.3500 fg-1 pg
ML265123.127.330.93235.7NA31.11 pg
ML266625.1283232.334.735.632.11 pg
  • a NA, not applicable.

  • b The transcription levels of 26 hypothetical unknown ORFs were similar to that of ML0049, an immunologically important antigen expressed at fairly low levels (22, 29).