Table 2

PUUV neutralizing antibodies and protection against PUUV infection

VaccineaHamster IDPrechallenge PUUV PRNT50 titerdPostchallenge N-ELISA titer (log10)b,dProtectionc
pWRG/PUU-M(s2)2695,1203N
2622,5601Y
261, 2631,2801Y
2436401Y
2483202N
2663201Y
245, 264, 2671601Y
242, 244, 249, 270801Y
241401Y
246, 250, 268201Y
26512N
None298403N
281, 284203N
282202N
28312N
285, 28613N
28714N
288, 290, 978, 98013N
29112N
292, 293–295, 29713N
pWRG/HTN-M(x)251, 252, 254, 258–260, 232, 236, 239, 24013N
253, 256, 231, 23714N
  • a Hamsters were vaccinated on weeks 0, 2, and 3 by use of ND10 delivery devices.

  • b Log10 endpoint titers represent GMTs for two N-ELISAs.

  • c A postchallenge N-ELISA titer of <2 indicates that the hamster was protected. N, not protected; Y, protected.

  • d A value of 1 indicates that the titer was below the limit of detection. The limit of detection for the PRNT was 20, and the limit of detection for the ELISA was 2 (log10).