Table 1

qRT-PCR analysis of 131 hypothetical unknown ORFs

ORFCT valuea for genomic DNACT valuea for cDNALevel of gene expression
1 ng100 pg10 pg1 pg500 fg100 fg50 fg10 fg
ML0009
ML0840
ML0939
ML2045
ML2669
ML046422.325.829.53233.53436.436.7<10 fg
ML092622.1303235.936.641NA<10 fg
ML114818.622.125.228.929.432.833.235.435.210 fg
ML047321.424.428.430.733.43436.434.810–50 fg
ML021833.236.736.338.138.73942.740.110–50 fg
ML029121.424.628.429.831.23334.534.310–50 fg
ML0293b21.224.828.830.431.132.736.83410–50 fg
ML093821.928.93031.332.23433.410–50 fg
ML100123.427.332.634.53638.736.510–50 fg
ML032525.6273233.134.737.935.410–100 fg
ML092726.430.833.934.237.54138.310–100 fg
ML015222.12629.530.432.534.135.6<50 fg
ML016223.727.932.440.63536.137<50 fg
ML047017.42124.628.729.731.732.136.4<50 fg
ML0659293439.541.642.8NA<50 fg
ML105738.939.6<50 fg
ML1210b19.523.527.43232.535.838.8NA<50 fg
ML160518.623.326.330.331.434NA<50 fg
ML191521.124.92932.433.635.736.637<50 fg
ML1976b20.424.227.931.932.534.535.6NA<50 fg
ML197919.42427.632.53334.935.336.9<50 fg
ML1989b19.623.627.731.8343636.5<50 fg
ML201319.723.827.931.133.235NA<50 fg
ML234633.534.136.136.5NA38<50 fg
ML2476b18.823.826.730.231.233.633.734.2<50 fg
ML247818.323.826.931.433.135.735NA<50 fg
ML24912429.533.537.939.5NA41.541.9<50 fg
ML256220.524.729.332.133.23536.137.1<50 fg
ML2603233035.63940.541.942.6NA<50 fg
ML262918.724.628.733.533.13736.9NA<50 fg
ML002424.628.331.6NA37.639NA38.150 fg
ML066422.426.130.434.134.334.550 fg
ML077722.12629.530.832.83535.550 fg
ML118818.522.526.13030.532.934.334.550 fg
ML118923.727.932.13336.13736.850 fg
ML129217.721.425.12930.2323333.650 fg
ML1420b1922.926.530.131.934.134.739.850 fg
ML176118.125.829.730.131.932.932.650 fg
ML20911923.42731.231.83234.234.250 fg
ML217023.528.531.936.136.838.539.341.239.450 fg
ML21722226.431.733.73638.5394339.650 fg
ML249720.926.730.334.8363940.540.450 fg
ML00253537.13839.442.541.1.50–100 fg
ML012632.73334.636.8NA35.650–100 fg
ML0292b20.824.427.728.731.932.634.63150–100 fg
ML036923.326.130.632.133.436.434.550–100 fg
ML086322.7262931323433.250–100 fg
ML09572325.929.73232.835.334.750–100 fg
ML101818.722.425.727.930.231.63432.550–100 fg
ML1243b23.92933.238.539.140.842.542.350–100 fg
ML127518.822.926.731.331.733.43634.150–100 fg
ML152318.622.426.729.830.833.434.734.550–100 fg
ML16021821.925.429.230.231.534.336.533.250–100 fg
ML176318.926.930.53133.5363450–100 fg
ML1788b18.822.52630.231.233.234.8393450–100 fg
ML182917.822.526.329.830.53132.732.550–100 fg
ML220118.923.327.13131.232.332.832.650–100 fg
ML095023.82831.13233.635.7<100 fg
ML096425.329.532.833.43536.6<100 fg
ML095023.82831.13233.635.7<100 fg
ML111920.324.329.233.233.236NA<100 fg
ML151721.425.930.934.236.438.8<100 fg
ML160417.92225.529.630.232.834.2<100 fg
ML1717b18.826.230.631.133.335<100 fg
ML18211822.425.729.733.634.440.7<100 fg
ML2452192427.93232.634.635.2<100 fg
ML226517.621.625.12929.4NANA31.3<100 fg
ML217821.425.931.234.635.937NA<100 fg
ML039434.8NA38.1NA39.438.5100 fg
ML066323.226.23131.93332.9100 fg
ML094922.63132.13536.136.8100 fg
ML10111821.82528.730.232.432.83532100 fg
ML11061922.826.429.931.333.638.833.3100 fg
ML1344b17.521.424.728.529.331.8NA34.3100 fg
ML215818.924.127.631.532.733.23533.6100 fg
ML226418.823.627.33233.335.436.235.5100 fg
ML24681822.125.229.53032.534.432.1100 fg
ML0472b21.325.328.231.932.834.432100–500 fg
ML065622.425.83030.834.33533.6100–500 fg
ML067918.522.425.929.530.533.433.831100–500 fg
ML094721.929.130.731.832.533.53932100–500 fg
ML118618.522.325.729.33135.134.5100–500 fg
ML129417.221.424.628.129.331.733.231100–500 fg
ML1572b21.525.829.730.432.633.531.7100–500 fg
ML16011822.225.329.23134.332.1100–500 fg
ML160318.12225.728.729.432.131100–500 fg
ML179318.721.926.530.83132.634.531.8100–500 fg
ML179618.322.82630.432.232.833.632.5100–500 fg
ML192817.521.82528.429.131.832.730.9100–500 fg
ML197219.323.426.630.632.134.435.533.3100–500 fg
ML2035b22.526.329.633.333.83635100–500 fg
ML217619.223.928.431.532.534.238.739.832.5100–500 fg
ML225219.924.327.23232.83637.941.135.5100–500 fg
ML228819.4242731.832.736.234.5100–500 fg
ML237918.824.327.73232.436.73733.7100–500 fg
ML262119.625.329.533.73637.93936.8100–500 fg
ML096323.429.832.735n/a<500 fg
ML0023b24.626.938.937.137.938.6NA37.4500 fg
ML012123.426.931.832.933.536.231.8500 fg
ML012725.329.932.733.234.3NANA33.5500 fg
ML0265b29.332.13539.142.735.4500 fg
ML07572326.73031.833.135.431.7500 fg
ML092828.83439404240.3500 fg
ML14451922.726.33032343632.4500 fg
ML228317.722.725.629.731.134.931500 fg
ML228419.22431.63234.335.234.2500 fg
ML044821.725.229.133.334.231.4500 fg-1 pg
ML057321.624.728.833.434.8344132500 fg-1 pg
ML075523.226.73131.833.135.431.7500 fg-1 pg
ML092023.827.431.132.53531.5500 fg-1 pg
ML09532729.533.835.139.234.1500 fg-1 pg
ML194919.623.727.131.432.934.132500 fg-1 pg
ML263017.322.826.530.931.632.9NA31.3500 fg-1 pg
ML0141b3637.639.240.642.7NA36.51 pg
ML018823.929.135.436.436.8NA34.61 pg
ML0217b23.828.433.936.937.140.4321 pg
ML007025.230.733.633.53434.235.433.61 pg
ML057423.12730.5313233.230.91 pg
ML058822.82630.531.832.734.830.11 pg
ML061420.82529.132.43637.5321 pg
ML0959242932.533.436.232.61 pg
ML101018.422.425.228.730.232.232.928.51 pg
ML138418.822.725.230.43134.835.230.71 pg
ML157526.431.134.437.838.941.437.91 pg
ML20442125.73033.734.73739.133.91 pg
ML230719.924.627.231.833.234.835.231.51 pg
ML231320.92630.534.335.838.732.31 pg
ML265119.323.127.330.93235.7NA31.11 pg
ML266619.525.1283232.334.735.632.11 pg
ML052722.425.429.230.232.433.228.9>1 pg
ESAT-6222629.431.732.435.229≈1 pg
ML03802831.536.732.11–10 pg
ML203820.22427.53132.93426.21–10 pg
  • a The CT (cycle threshold) value is the number of cycles at which the fluorescence signal generated by PCR just exceeds the background fluorescence level (threshold) reached at the exponential phase of the PCR (http://www3.appliedbiosystems.com/cms/groups/mcb_marketing/documents/generaldocuments/cms_042502.pdf). NA, not applicable.

  • b Recent gene annotation analysis revealed that the gene is a pseudogene or doubtful CDS. The expression level of each gene was determined by comparison to the results of qRT-PCR assays performed using a series of genomic DNA standards.