Table 1

Characteristics of bacterial strains and primers used in this study

Strain, plasmid, or primerRelevant characteristics or sequenceSource or reference
Strains
    4074A. pleuropneumoniae reference strain of serovar 1, apfA positiveP. Blackall
    WF83A. pleuropneumoniae reference strain of serovar 7, apfA positiveP. Blackall
    4074ΔapfAA. pleuropneumoniae 4074 apfA mutation strainThis work
    C4074ΔapfAA. pleuropneumoniae apfA complementary strainThis work
Plasmids
    pET-30a(+)Expression vector, kanamycin resistantNovagen
    pJN105-sodCShuttle vector pJN105 with a sodC promoter, gentamicin resistant17
    pEMOC2Suicide vector, chloramphenicol resistant18
Primers
    apfA015′-CGTACGGTCGACGAATTGATGATCGTGATTGC-3′This work
    apfA025′-CGCTAGGCGGCCGCTAAGTCCTCCCCTTTACATT-3′This work
    apfA035′- CGTACGGAATTCATGCAAAAACTAAGTCTTATTC-3′This work
    apfA045′- CGCTAGTCTAGATTAATTTGATGCGCAGAAATT-3′This work
    apfA055′-CGTACGGAATTCTTTACTTTAATTGAATTGATGAT-3′This work
    apfA065′-CGCTAGGTCGACTTAATTTGATGCGCAGAAATT-3′This work
    apfA-S5′-CGAGATCTGCATATATAACACCG-3′This work
    apfA-A5′-CAGAAATTTGCCGGAAATAAAC-3′This work
    apfB-S5′-TTCGGGAAGACCAACAAACA-3′This work
    apfB-A5′-TAAACAGGCCGTCCAACACT-3′This work
    apfC-S5′-AACAAAGCGGGCGATTAGG-3′This work
    apfC-A5′-GGCACGATAAAAAGGAGCATG-3′This work
    ackA-S5′-GCGGAAGATAACTACGAGGACG-3′This work
    ackA-A5′-AATGTTGAGTCGTCCGCAGTG-3′This work