Table 1

Comparison of differences in deduced M segment open reading frame amino acids for four strains of PUUV and three DNA vaccine constructsf

Amino acid positionaPUUV M segment amino acidDNA vaccine amino acid
DTK/Ufa-97bK27cP360dHallnas B1ePUU-M(x22)PUU-M(s1)PUU-M(s2)
10YYCYYYY
38VIIIIII
187QQQDQQQ
207HHHHYYH
312AAAVAAA
416LSLLLLL
448MMMMTTM
492WWWWRRW
501FLFFFFF
604TTTRTTT
649LLLHLLL
650EEEHEEE
675GGGRGGG
690PPPQPPP
972QQQQRRQ
1077GGGPGGG
1097SSSSLLS
  • a M gene segment open reading frame amino acid position.

  • b DTK/Ufa-97, PUUV strain DTK/Ufa (GenBank accession no. BAF49040).

  • c K27, PUUV strain K27 (GenBank accession no. P41265).

  • d P360, PUUV strain P360 (GenBank accession no. P41266).

  • e Hallnas B1, PUUV strain Hallnas B1 (GenBank accession no. P21400).

  • f Amino acids shown in bold are differences with respect to the PUUM(s2) sequence.