Table 1

Peptides used in this study and their diagnostic propertiesa

Peptide designationPeptide sequenceProteinSensitivity (%)Specificity (%)Cutoff valueReference or source
Peptides from literature
    SAG1-48SDPPLVANQVVTCPDKKSSAG14.353.571.8726
    dSAG1-62DKKSTAAVILTPTENHFTSAG13.263.571.6226
    SAG1-76NHFTLKCPKTALTEPPTLASAG12.723.572.8126
    SAG1-90PPTLAYSPNRQICPAGTTSSAG13.263.572.0326
    SAG1-104AGTTSSCTSKAVTLSSLIPSAG11.633.571.2926
    SAG1-146PIEKFPVTTQTFFWGCIKGSAG13.263.571.3026
    SAG1-273AFPAESKSVIIGCTGGSPESAG12.173.571.8426
    SAG1-287GGSPEKHHCTVKLEFAGAASAG15.433.570.5426
    dSAG1-301FAGAAGSAKSAAGTASHVSSAG11.093.572.2826
    SAG1-315ASHVSIFAMVIGLIGSIAACVASAG116.853.571.1326
    SAG1-61DKKSTAAVILTPTENHFTLSAG19.783.571.5311
    SAG1-181SVVNNVARCSYGADSTLGPSAG11.633.572.0611
    SAG1-241SFKDILPKLTENPWQGNASSAG12.723.572.6011
    SAG1-261DKGATLTIKKEAFPAESKSSAG12.173.571.4811
    SAG1-301AGAAGSAKSAAGTASHVSISAG10.543.571.7111
    GRA1-100EEVIDTMKSMQRDEDGRA12.723.572.816
    NTP1CKAPMIVTGGGMLAA INTLKNTP14.353.571.8225
    NTP2APMFITGREMLASIDTLKNTP23.263.571.9125
Novel peptides
    GRA1-162MKVIDDVQQLEKDKQQGRA134.243.572.83This study
    GRA2-61DERQQEPEEPVSQRASGRA230.983.571.28This study
    GRA2-28GVVNQGPVDVPFSGKPGRA250.003.570.98This study
    GRA2-67PEEPVSQRASRVAEQLGRA24.893.573.39This study
    GRA2-38PFSGKPLDERAVGGKGGRA29.243.571.71This study
    GRA4-300GGTRTSTAPAEAGKTEGRA49.243.571.06This study
    GRA4-316LDDGYRPPPFNPRPSPGRA44.353.570.95This study
    GRA4-309PAEAGKTELDDGYRPPGRA433.153.571.13This study
    GRA4-321PPPFNPRPSPYAELLKGRA413.043.572.33This study
    MIC3-219GETLVNLPEGGQGCKRMIC32.723.573.19This study
    MIC3-15SGAVWMCTPAEALPIQMIC30.003.573.08This study
    MIC3-88RQLHTDNGYFIGASCPMIC31.093.572.82This study
    MIC3-324GSEGSLSEKMNIVFKCMIC310.333.571.37This study
    MIC3-238HAFRENCSPGRCIDDAMIC32.173.572.64This study
    MIC3-282GVEVTLAEKCEKEFGIMIC319.573.572.82This study
    MIC3-191SKRGNAKCGPNGTCIVMIC331.523.571.22This study
    MIC3-26ALPIQKSVQLGSFDKVMIC32.173.571.66This study
    MIC3-291CEKEFGISASSCKCDNMIC37.613.571.73This study
    MIC3-8LLHALTFSGAVWMCTPMIC31.633.572.25This study
    MIC3-39DKVVPSREVVSESLAPMIC32.173.571.26This study
  • a Peptide sequences were either previously published (n = 18) or selected after in silico epitope prediction (n = 20). The number at the end of the peptide names indicates the position of the first amino acid of the peptide in the protein sequence. The individual cutoff value for each peptide was selected using the mean index values obtained for a panel of 140 T. gondii-seronegative human sera and accepting a maximum of 4% false-positive reactions.