Table 1

Properties of pertussis toxin antigens from the four sourcesa

Property of PT antigenList BiologicalsProtein ExpressGlaxoSmithKline BiologicalsSanofi Pasteur
Production strain165Tohama ITohama I10536
Purity (%)Not available>99>9599.5
BioactivityCHO cell assayCHO cell assayCHO cell assayCHO cell assay
Purity assessment method15% SDS-PAGE13% SDS-PAGESDS-PAGESDS-PAGE/densitometry PT bands
Storage buffer components50% Glycerol50% Glycerol50% Glycerol50% Glycerol
0.05 M Tris (pH 7.5)0.05 M Tris-Cl (pH 7.6)25 mM Phosphate buffer (pH 7.6)37.5 mM Potassium phosphate (pH 8.0)
0.5 M NaCl0.5 M NaCl250 mM NaCl112.5 mM NaCl
0.01 M Glycine
Storage temp (°C)−20−20−202–8
  • a PT, pertussis toxin; CHO, Chinese hamster ovary; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis.