Table 1.

Bacterial strains and plasmidsa

Strain or plasmidRelevant genotype, phenotype, or descriptionbSource or referencec
Strains
    DH5αFor general DNA manipulations and detection of bacteriophageStratagene
    TG1For general DNA manipulations and detection of bacteriophageStratagene
    SM10λpirpir traRP433, IAH
    WS-2773EO141:H5 CS5 CS6 LT ST EAST1 (clinical isolate)NMRC
    ACAM2006O141:H5 CS5 CS6 ΔastA ΔeltAB ΔestA ΔompC ΔompF ΔaroCThis work
    ACAM2012O141:H5 CS5 CS6 ΔastA ΔeltAB ΔestA ΔompC ΔompF ΔaroC ΔphageThis work
    ACAM2022O141:H5 CS5 CS6 ΔastA ΔeltAB ΔestA ΔompF::Ptac-LTB ΔaroC ΔphageThis work
    WS-3504DO39:H12 CS2 CS3 LT ST EAST1 Apr (clinical isolate)NMRC
    ACAM2007O39:H12 CS2 CS3 ΔastA ΔeltAB ΔestA ΔompC ΔompF ΔaroCThis work
    ACAM2017O39:H12 CS2 CS3 ΔastA ΔeltAB ΔestA ΔompC::CS1 ΔompF ΔaroCThis work
    ACAM2027O39:H12 CS2 CS3 ΔastA ΔeltAB ΔestA ΔompC::CS1 ΔompF::Ptac-LTB ΔaroCThis work
    E1392/75O6:H16 CS1 CS3 ST LT Sur Smr5
    E1392/75-2AO6:H16 CS1 CS3 Sur Smr5
    PTL003O6:H16 CS1 CS3 Sur Smr ΔaroC ΔompC ΔompF32
    ACAM2008O6:H16 CS1 CS3 ΔaroC ΔompC ΔompFThis work
    WS-1858BO71:H CFA/I ST EAST1 Apr Tpr Sur (clinical isolate)NMRC, 31
    ACAM2010O71:H CFA/I ΔastA ΔestA ΔaroC ΔompC ΔompF31
    ACAM2025O71:H CFA/I ΔastA ΔestA ΔaroC ΔompC ΔompF::pLLTBThis work
Plasmids
    RP460-kb plasmid, covalently closed circular DNA size standard31
    pvirF98∼90-kb plasmid from Salmonella enterica serovar Typhimurium F98, covalently closed circular DNA size standard31
    ColVIk94∼127-kb plasmid, covalently closed circular DNA size standard31
    pKK223-3Expression vector, source of Ptac and rrnB transcription terminatorAmersham-Pharmacia
    pPCR-Script Amp SK+Plasmid vectorStratagene
    pPCRPromNative LT promoter in pPCR-ScriptThis work
    pACYC184Plasmid vector3
    pACYC-Tcori15A Tcr31
    pACYC-CS1CS1 operon in pACYC184This work
    pACYC-csaACS1 csaA gene in pACYC184This work
    pACYC-PtacLTBPtac-LTB construct in pACYC184This work
    pACYC-PtacLTBrrnBPtac-LTB-rrnB transcription terminator construct in pACYC184This work
    pACYC-ΔompCThis work
    pACYC-ΔompC::cooBAThis work
    pJCB12oriR6K mobRP4 sacB cat31
    pJCB12-ΔeltA658-bp deletion construct of LTA gene in pJCB12This work
    pJCB12- ΔestAEST gene deletion fragment in pJCB12This work
    pJCB12-ΔastA286-bp fragment with 42-bp defined deletion in EAST1 gene in pJCB1231
    pJCB12-ΔompC1,016-bp fragment with deletion of the whole ompC gene in pJCB1231
    pJCB12-ΔompF943-bp fragment with deletion of the whole ompF gene in pJCB1231
    pJCB12-ΔompF::LTBThis work
    pJCB12-ΔompF::PtacLTBThis work
    pJCB12-ΔompF::PtacLTBrrnBLTB expressed from tac promoter, incorporating rrnB transcriptional terminator and flanked by ΔompF nucleotide sequences in pJCB12This work
    pJCB12-ΔompF::LLTBThis work
    pJCB12-ΔompC::CS1CS1 operon flanked by ΔompC nucleotide sequences in pJCB12This work
    pJCB12-ori15ApACYC184 replication origin in pJCB1231
    pTETnir15Inducible expression vector4
    pLLTBpTETnir15 expressing LTB from its native promoterThis work
  • a For the sake of brevity, some strains are not listed.

  • b Antibiotic resistance: Nalr, nalidixic acid; Apr, ampicillin; Tpr, trimethoprim; Smr, streptomycin; Sur, sulfamethoxazole; Tcr, tetracycline.

  • c IAH, provided by P. Barrow, Institute for Animal Health, Compton, United Kingdom; NMRC, provided by S. Savarino, Naval Medical Research Center, Silver Spring, MD.