Table 1.

Bacterial strains and recombinant plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
    H10407Wild-type ETEC strain; serotype O78:H11; CFA/I LT+ STh+ STp+9
    jf1412fliC mutant of H10407; nonmotile; Kmr30
    Top10F mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL StrrendA1 nupGInvitrogen
    jf876ΔlacZYA derivative of H104078
    BL21(DE3)FompT hsdSB(rB mB)dcm gal (DE3)
Plasmids
    pJL017etpBA cloned into pBAD/Myc-HisA, with etpA in frame with Myc and His6 coding regions28
    pJL030etpC gene cloned into pACYC184; Cmr30
    pGPM1034cexE gene cloned into pET33b in frame with His6 coding region24