Table 3.

Representative dilution series for HBV genotypes A to Ea

GenotypeUndilutedDilution
1:100*1:400†1:1,0001:4,0001:10,0001:40,0001:100,0001:1,000,000
A123.40>130‡33,05232,64032,12031,80031,24030,300<0.05#
B>130‡1,1931,1481,2201,1961,340<0.05#<0.05#<0.05#
C>130‡673728716744740<0.05#<0.05#<0.05#
D33.31>130‡>130‡78,41077,12080,90077,20078,10078,000
E>130‡>130‡24,08023,80024,00023,90024,44023,400<0.05#
  • a Onboard dilution for Elecsys 2010 or cobas e 411 (*) and Modular Analytics E170, cobas e 601, or cobas e 602 (), respectively. The mandatory dilution algorithm incorporating onboard dilution eliminates any potential high-dose hook effect. >130‡ indicates that the HBsAg level in the diluted sample is above the upper limit of detection for the assay; therefore, the level in the undiluted sample cannot be calculated. <0.05# indicates that the HBsAg level in the diluted sample is below the lower limit of detection for the assay; therefore, the level in the undiluted sample cannot be calculated.