Table 3.

Specificity of IDEXX M. bovis antibody ELISA with sera collected from cattle naturally infected with M. avium subsp. paratuberculosis

Sample IDcM. avium subsp. paratuberculosis detectionM. bovis ELISA (S/P)b
Fecal cultureAntibody ELISA (S/P)aLot 1Lot 2Lot 3
116Positive2.53−0.08−0.18−0.04
164Positive3.29−0.05−0.17−0.04
179Positive1.43−0.08−0.17−0.04
194Positive2.380.03−0.05−0.03
211Positive2.33−0.26−0.16−0.04
222Positive3.35−0.09−0.16−0.05
229Positive0.08−0.10−0.19−0.05
232Positive1.860.05−0.140.00
253Positive3.20−0.09−0.18−0.05
263Positive2.77−0.09−0.17−0.06
270Positive3.33−0.07−0.17−0.06
275Positive1.45−0.08−0.13−0.04
289Positive2.04−0.11−0.19−0.04
290Positive2.600.01−0.090.00
305Positive3.22−0.08−0.15−0.04
307Positive1.60−0.09−0.17−0.06
319Negative0.63−0.26−0.14−0.04
323Positive0.10−0.09−0.16−0.05
324PositiveNA−0.10−0.19−0.06
  • a M. paratuberculosis antibody ELISA was performed according to the manufacturer's instructions (IDEXX Laboratories). The antigen preparation in this kit is a complex protoplasmic antigen. All responses were considered positive except those for animals 229 and 323. NA, not available for testing.

  • b All responses were considered negative.

  • c ID, identification.