TABLE 2.

Optimized concentrations of reagents

CytokineCapture antibody (μg/106 microspheres)Detection antibody (ng/ml)Streptavidin-PE (μg/ml)Microsphere input (per region per well)
IFN-γ52,40032,500
IL-10520032,500
IL-12/IL-23 p40530032,500