TABLE 1.

Primary antibodies used for this studya

AntigenMAb cloneIsotypeWorking MAb concnb (μg/ml)Specificity
CD4GC50A1IgM14T-helper cell
CD5B29aIgG2a7Activation marker
CD8BAQ111AIgM14T-cytotoxic/suppressor cell
CD25CACT116AIgG115IL-2 receptor
CACT108AIgG2a15
LCTB2AIgG315
CD26CACT114AIgG2b15Activation marker
CD45ROGC42A1IgG110Memory/activation marker
N12CACT61AIgM14γδ-Cell receptor
  • a Obtained from VMRD Inc. (Pullman, WA).

  • b Diluted in PBS with 1% fetal calf serum and 0.04% sodium azide.