Table 1.

PCR primers used in this study

Amplified regiona and primer namePrimer sequencebRestriction enzyme
CAT (1223–1521)
    GtfB/CAT-F5′-TTAGGAATTCCTTATGGCTAACGATGTGG-3′EcoRI
    GtfB/CAT-R5′-CCGCTCGAGATCATATTGTCGCCATCATC-3′XhoI
GBD (3238–4431)
    GtfB/GBD-F5′-GGTCGCGGATCCGTTTATTATCAACGAGTGG-3′BamHI
    GtfB/GBD-R5′-AGCGGGAATTCCATTAGTTAATCCGAAC-3′EcoRI
VR (115–1216)
    GtfB/VR-F5′-GTTGGTACCGATTCTAATGAATCGAAATC-3′KpnI
    GtfB/VR-R5′-AACTGCAGTTCGTAACCGCCGATAG-3′PstI
  • a The numbers in parentheses indicate the amplified nucleic acid positions, from the 5′ start of gtfB.

  • b The bold characters in the primer sequences indicate the added restriction enzyme site.