Table 1.

Comparison of the BioPlex MMRV IgG assays to routine testing by EIA

Assay and resultNo. of samples by EIA that were:% sensitivity (95% CI)% specificity (95% CI)% agreement (95% CI)Kappa value
PositiveNegativeEquivocal
BioPlex measles IgG (against Diamedix EIA)94.6 (92.1, 96.4)96.4 (80.8, 100)91.6 (88.8, 93.8)0.66
    Positive4201a0
    Negative10b2717
    Equivocal14011
BioPlex mumps IgG (against SeraQuest EIA)98.1 (96.2, 99.1)82.8 (70.9, 90.6)94.2 (91.8, 96.0)0.79
    Positive4124c8
    Negative3d483
    Equivocal5611
BioPlex rubella IgG (against SeraQuest EIA)94.9 (92.5, 96.6)100.0 (83.1, 100)94.4 (92.0, 96.1)0.65
    Positive44600
    Negative7e234
    Equivocal1703
BioPlex varicella-zoster virus IgG (against Diamedix EIA)92.2 (89.4, 94.3)100.0 (82.5, 100)91.8 (89.0, 93.9)0.52
    Positive43600
    Negative18f224
    Equivocal1901
  • a This sample tested negative by the SeraQuest IgG EIA.

  • b All 10 samples tested positive by the SeraQuest IgG EIA.

  • c All four of these samples tested positive by Vidas mumps IgG ELFA.

  • d One of these three samples tested negative by Vidas mumps IgG ELFA.

  • e Six of these seven samples tested as equivocal by Vidas rubella IgG ELFA.

  • f All 18 samples tested positive by the SeraQuest IgG EIA.