Table 5.

Optimization of ELISA performance using combinations of MAbs F12D2 and 339

MAbs used for ELISA constructionaMinimum concn of GXM (ng/ml) producing a positive resultb
ABCD
Capture MAb(s)Indicator MAb(s)CN6MU-11844093429824066127M0024
F12D2F12D20.470.290.780.806.82.12.32.83.2
F12D23391.10.703.75.01,700220>2,0000.931.1
F12D2F12D2/3390.640.431.11.29.52.82.80.911.0
339F12D20.820.510.941.12,000981,5002.72.4
3393390.831.09.711>2,0001,100>2,0001.00.65
339F12D2/3391.00.622.02.0>2,0001551,9000.970.61
F12D2/339F12D20.460.270.740.756.61.92.02.42.8
F12D2/3393391.20.713.64.42,000420>2,0000.670.51
F12D2/339F12D2/3390.760.440.850.86102.72.80.630.51
  • a The indicated MAb or a 50:50 mixture of the two MAbs was used in either the capture phase of the ELISA or as a HRPO conjugate (indicator MAb[s]).

  • b Results are shown for GXM isolated from different strains of each serotype. >2,000, GXM of some strains of serotype C at concentrations of 2,000 ng/ml failed to produce an OD of 0.5.