Table 1.

Reactive clones by immunoscreening of Mycoplasma haemofelis Lambda ZapII libraries and BLASTx results against nonredundant protein sequence database

Insert identificationGenBank accession no.Sequence similarity by BLASTx against the MollicutesE value
P3-orf1165a,bGS928052ACQ84443.1, adhesin (Mycoplasma hyopneumoniae)0.002
P5-orf1816a,b,cGS928053NP_853366.1, thymidine phosphorylase (M. gallisepticum)0.37
P6D-orf0908a,b,dGS928054ZP_06610317.1, hypothetical protein MALL_0643 (M. alligatoris)0.44
P6D-orf0909bGS928055ZP_06610593.1, hypothetical protein MALL_0515 (M. alligatoris)0.63
P7-orf0259GS928056ACU78513.1, triose-phosphate isomerase (M. mycoides)8E-36
P7-orf0260GS928057NP_073101.2, phosphoglyceromutase (M. genitalium)9E-128
P7-orf0261GS928058BAH70152.1, hypothetical protein (M. fermentans)0.026
P7-orf0262aGS928059NSe
P7-orf0263aGS928060ABD47695.1, adhesin-like protein P146 (M. hyopneumoniae)1.4
P7-orf0264GS928061AAZ44718.2, conserved hypothetical protein (M. hyopneumoniae)0.22
P9D-orf1202GS928062ZP_06610215.1, type I restriction modification DNA protein (M. alligatoris)2E-15
P9D-orf1203GS928063YP_003303059.1, type I restriction enzyme specificity protein (M. hominis)0.005
P9D-orf1204GS928064ZP_02931536.1, type I restriction enzyme S protein (Ureaplasma parvum)4E-10
P9D-orf1205GS928065YP_003303059.1, type I restriction enzyme specificity protein (M. hominis)2E-12
P10B-orf1747a,bGS928066YP_002284694.1, putative lipoprotein (Ureaplasma urealyticum)3.1
P10B-orf1748GS928067YP_003515875.1, hypothetical protein MAGa7180 (M. agalactiae)0.17
P10B-orf1749a,bGS928068NS1.1
P10B-orf1750GS928069YP_003560289.1, hyaluronoglucosaminidase (M. crocodyli)1.1
P10C-orf1238a,b,dGS928070YP_002000188.1, massive surface protein MspK (M. arthritidis)0.004
P10C-orf1239a,b,dGS928071ZP_04563878.1, transcriptional regulator (Mollicutes bacterium D7)0.002
P10E-orf0279a,dGS928072NP_757929.1, hypothetical protein MYPE5440 (M. penetrans)3E-50
P10E-orf0280GS928073NP_975564.1, pseudouridylate synthase D (M. mycoides)1E-41
P15-orf0941GS928074YP_002960937.1, hypothetical protein MCJ_004270 (M. conjunctivae)0.0024
P15-orf0942GS928075NS
P15-orf0943bGS928076ACU78785.1, conserved hypothetical protein (M. mycoides)0.28
P15-orf0944GS928077AAO39838.1, AvgC variable lipoprotein (M. agalactiae)0.17
P15-orf0945a,bGS928078YP_002000023.1, massive surface protein MspF (M. arthritidis)0.63
P15-orf0946a,bGS928079ZP_02695921.2, hypothetical protein UUR13 (U. urealyticum)1.8
P15-orf0947a,bGS928080ZP_06610731.1, conserved hypothetical protein (M. alligatoris)0.37
P15-orf0948bGS928081YP_279005.1, lysyl-tRNA synthetase (M. hyopneumoniae)0.63
P15-orf0949bGS928082NP_758309.1, phenylalanyl-tRNA synthetase subunit beta (M. penetrans)0.48
P17A-orf1526GS928083CAB62239.1, P75 protein (M. hominis)0.28
P17A-orf1527a,bGS928084ZP_04564868.1, conserved hypothetical protein (Mollicutes bacterium D7)0.002
P17A-orf1528bGS928085ZP_02971377.1, conserved hypothetical protein (U. parvum)0.37
P17A-orf1529bGS928086YP_016078.1, hypothetical protein MMOB3810 (M. mobile)0.37
P18-orf0127GS928087NP_757967.1, hypoxanthine-guanine phosphoribosyltransferase (M. penetrans)6E-07
P18-orf0128aGS928088YP_002000162.1, hypothetical protein MARTH (M. arthritidis)0.13
P20/22-orf0326aGS928089NP_757466.1, DNA-directed RNA polymerase subunit beta (M. penetrans)0
P21A-orf0675a,bGS928090NP_757933.1, translocase (Mycoplasma penetrans)3.1
P21B-orf1544a,bGS928091YP_002000128.1, massive surface protein MspH (M. arthritidis)0.044
P21B-orf1545GS928092NP_757749.1, hypothetical protein MYPE3620 (M. penetrans)0.057
P21B-orf1546a,b,dGS928093YP_001256183.1, hypothetical protein MAG_0390 (M. agalactiae)0.097
P21B-orf1547bGS928094NP_975173.1, hypothetical protein MSC_0170 (M. mycoides)1.1
P21B-orf1548bGS928095YP_001799373.1, hypothetical protein PAa (C. Phytoplasma australiense)0.13
P24-orf1679aGS928096YP_002000015.1, massive surface protein MspC (M. arthritidis)1.13
P24-orf1680GS928097NS
P24-orf1681bGS928098NP_758083.1, hypothetical protein MYPE6950 (M. penetrans)0.28
P26-orf0285GS928099NP_853008.2, translation elongation factor Tu (EF-Tu) (M. gallisepticum)8E-129
P26-orf0286GS928100NP_757969.1, adenylosuccinate synthetase (M. penetrans)4E-110
P26-orf0287GS928101ADC31594.1, ribosomal biogenesis GTPase (M. gallisepticum)2E-31
P27A-orf1350bGS928102YP_002000022.1, massive surface protein MspE (M. arthritidis)2E-03
P28-orf1521a,bGS928103YP_002961131.1, hypothetical protein MCJ_006330 (M. conjunctivae)0.130
P28-orf1522bGS928104YP_016010.1, SWF/SNF family helicase (M. mobile)0.630
P28-orf1523bGS928105ZP_04563267.1, conserved hypothetical protein (Mollicutes bacterium D7)0.220
P29-orf0175GS928106YP_001621362.1, ketose bisphosphate aldolase (Acholeplasma laidlawii)5E-102
P29-orf0176GS928107NP_072863.1, cochaperone GrpE (M. genitalium)4E-22
P29-orf0177aGS928108NP_758284.1, heat shock protein DnaJ (M. penetrans)2E-69
P29-orf0178GS928109NP_109915.1, elongation factor G (M. pneumoniae)0
P32C-orf0088GS928110ZP_03079605.1, arginyl-tRNA synthetase (U. urealyticum)7E-75
P33B-orf1097GS928111YP_002000188.1, massive surface protein MspK (M. arthritidis)0.057
  • a Putative proteins selected by bioinformatics analyses to be cloned and expressed by the Gateway system.

  • b Putative proteins predicted to have a transmembrane domain(s) by hidden Markov model and dense alignment surface methods.

  • c Shading indicates ORFs within the same plaque.

  • d Putative proteins that were reactive against immune plasma and negative against nonimmune plasma by Western blotting.

  • e NS, no significant similarity found.