Table 2.

BD reagents used and peripheral blood lymphocyte subsets measured in the study

Reagent (BD)SubsetAnchor markerSurface markeraSubset
Multitest CD3/CD19/CD45/CD16 + 5619CD453/19B cell
16/56CD453/natural killerNatural killer cell
Multitest CD3/CD8/CD45/CD44CD453/4Helper T cell
8CD453/8Cytotoxic T cell
3CD45T cells
Multitest CD45RA/CD62L/CD3/CD44/45RA/62LCD445RA/62LNaive helper T cell (CD62L+)
4/45RACD445RA/62L+62LNaive helper T cell
Multitest CD45RA/CD62L/CD3/CD88/45RA/62LCD845RA/62LNaive cytotoxic T cell (CD62L+)
8/45RACD845RA/62L+62LNaive cytotoxic T cell
Multitest CD4/CD38/CD3/HLA-DR4/DR/38CD4DR/38Activated helper T cell
4/DRCD4DR/38+38Activated helper T cell (DR+)
CD438/DR+DRActivated helper T cell (CD38+)
Multitest CD8/CD38/CD3/HLA-DR8/DR/38CD8DR/38Activated cytotoxic T cell
8/DRCD8DR/38+38Activated cytotoxic T cell (DR+)
CD838/DR+DRActivated cytotoxic T cell (CD38+)
Multitest CD45RA/CD45RO/CD3/CD43/4/45ROCD34/45ROMemory helper T cells
3/4/45RACD34/45RANaive helper T cells
Multitest CD45RA/CD45RO/CD3/CD83/8/45ROCD38/45ROMemory cytotoxic T cells
3/8/RACD38/45RANaive helper T cells
αβ/γδ/CD3γδ T cellCD3γδ+/αβγδ T cell
αβ T cellCD3γδ/αβ+αβ T cell
  • a The presence of a surface marker on a cell is assumed unless the marker is followed by a superscript minus sign. The presence of both plus and minus signs indicates a total cell population.