TABLE 2.

E. coli strains and plasmids used in this study

Strain or plasmidRelevant property(ies)Source or reference
Strains
    TOP10F mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 deoR araD139Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
    8851TOP10/pK88ac-LTB@EP3This study
    8549TOP10/pK88ac-LTB@EP4This study
    8616TOP10/pK88ac-STa@EP3This study
    8576TOP10/pK88ac-STa@EP3-LTB@EP4This study
    8069TOP 10/p8069 (K88ac)44
    3030-2Porcine ETEC isolate: K88ac LT STb12
Plasmids
    pBAD88-102K88ac operon in pBAD3
    p8069pBAD88-102 with SpeI site downstream of faeG gene44
    pK88ac-LTB@EP3K88ac with LTB epitope at EP3 of FaeG major subunitThis study
    pK88ac-LTB@EP4K88ac with LTB epitope at EP4 of FaeG major subunitThis study
    pK88ac-STa@EP3K88ac with STa epitope at EP3 of FaeG major subunitThis study
    pK88ac-STa@EP3-LTB@EP4K88ac with STa epitope at EP3 and LTB epitope at EP4 of FaeG major subunitThis study