TABLE 1.

PCR primers used to construct chimeric faeG major subunit genes of K88ac fimbriaea

PrimerSequence (5′-3′)
Eco81IG-FGTG AGG ATG TGG TGA TTA CCG TGC CTG AGG C
SpeIG-RTTA TTG ATA CGT TCT GAT AAC AGA CTA GTA TC
K88ac3:LTb-FTAT CGC AAC ACA CAA ATA TAT ACG ATA AAT GGT TTA GCA TAT TTT GTT
K88ac3:LTb-RTTG TGT GTT GCG ATA TTC CGA ACA TAG TAC TAC AGA AGC TCC TTC
K88ac4:LTb-FTAT CGC AAC ACA CAA ATA TAT ACG ATA AAT AAA GTG AAT GCA TCT TAT GCC
K88ac4:LTb-RTTG TGT GTT GCG ATA TTC CGA ACA TAG CGG CAG AAC AAC AAA ATA TGC TA
K88ac3:pSTa13-FTGT TGT AAT CCT CAT TGT GCT GGA TGT TAT GGT TTA GCA TAT TTT GTT
K88ac3:pSTa13-RACA CTG AGG ATT ACA ACA AAG TTC ACA GCA TAC TAC AGA AGC TCC TTC
  • a Nucleotides underlined represent restriction enzyme sites, and nucleotides in italics are of the LTB epitope or the STa13 epitope.