Assay | PBMC prep | Group (no. of samples) | Median antigenic stimulus for: | ||
---|---|---|---|---|---|
Gag pool | PEN pool | p24 | |||
ICC-flow IFN-γ CD8^{+} | Fresh | A (17) | <5 | 6.3 | <5 |
Fresh | B (14) | 23.2 | 33.9 | 8.7 | |
P value | 0.048 | 0.019 | 0.14 | ||
Cryopreserved | A (11) | 5.5 | 21.9 | <5 | |
Cryopreserved | B (10) | 196.7 | 112.5 | <5 | |
P value | 0.036 | 0.042 | 0.31 | ||
ICC-flow IL-2 CD8^{+} | Fresh | A (17) | <5 | <5 | <5 |
Fresh | B (14) | <5 | <5 | 20.7 | |
P value | 0.61 | 0.80 | 0.36 | ||
Cryopreserved | A (11) | 42.7 | 30.8 | <5 | |
Cryopreserved | B (10) | 58.6 | 33.6 | 33.0 | |
P value | 0.47 | 0.76 | 0.07 | ||
ICC-flow IFN-γ CD4^{+} | Fresh | A (17) | <5 | <5 | <5 |
Fresh | B (14) | <5 | <5 | 11.4 | |
P value | 0.31 | 1.0 | 0.25 | ||
Cryopreserved | A (11) | 23.7 | <5 | <5 | |
Cryopreserved | B (10) | 23.9 | 11.5 | 9.2 | |
P value | 0.82 | 0.52 | 0.67 | ||
ICC-flow IL-2 CD4^{+} | Fresh | A (17) | <5 | <5 | <5 |
Fresh | B (14) | <5 | 10.4 | 6.2 | |
P value | 0.66 | 0.70 | 0.60 | ||
Cryopreserved | A (11) | 49.9 | 18.3 | <5 | |
Cryopreserved | B (10) | 27.1 | 11.9 | 79.7 | |
P value | 0.63 | 0.97 | 0.29 |
↵ a For each assay, data were averaged over 1 to 3 separate time points for each individual and each antigen, and medians are for the two arms tested for differences by the Wilcoxon rank sum tests. Comparisons that reached statistical significance are in bold.