TABLE 1.

Differential expression of genes for selected cytokines and chemokines after stimulation with the recombinant A. fumigatus antigens Aspf1 and Crf1a

GeneDifferential expression (fold) at the indicated times after stimulation with:
Aspf1Crf1
6 h24 h48 h6 h24 h48 h
TNF-α8.212223
IL-830722752.12.82.9
IL-23p197.52.2NDNDND
CXCL103960272.32.93.1
CCL202472993143.1
IL-12p35ND14NDNDNDND
IL-100.60.8NDNDND
IL-1βND7.4NDNDNDND
  • a DCs were stimulated for 6, 24, or 48 h with A. fumigatus antigens; and the levels of expression of the genes for TNF-α, IL-8, IL-23, CXCL10, and CCL20 were determined by quantitative reverse transcription-PCR. The level of gene expression was normalized against the level of expression of h-ALAS by evaluating the crossing point values for each sample by using the equation 2(ΔCP for target gene − ΔCP for h-ALAS), where ΔCP is the change in the crossing point (CP) value (7, 22). For each time point, the levels of gene expression by DCs cocultivated with A. fumigatus antigens were compared to the levels of gene expression by unstimulated cells (and are given as ratios). All experiments were repeated with blood from at least three independent blood donors, and the mean level of upregulation is indicated. —, unchanged expression, defined as a <2-fold change and a >0.8-fold change; ND, not determined.