TABLE 5.

Specificity of the anti-HBs in-house assay compared to the AUSAB EIA

AUSAB EIA antibody concn (mIU/ml) before preincubationAnti-HBs in-house assay antibody concn (mIU/ml) before preincubationHBsAg preincubation with subtype(s)a:
ad + ayadw2
AUSAB EIAAnti-HBs in-house assayAUSAB EIAAnti-HBs in-house assay
Antibody concn (mIU/ml)% SpecificityAntibody concn (mIU/ml)% SpecificityAntibody concn (mIU/ml)% SpecificityAntibody concn (mIU/ml)% Specificity
2015<3.3100<3.3100<3.3100<3.3100
9461<3.3100<3.3100<3.3100<3.3100
119125<3.3100<3.3100<3.3100<3.3100
346352<3.3100<3.3100<3.3100<3.3100
382428<3.3100<3.3100<3.3100<3.3100
1,5561,111<3.3100<3.3100<3.3100<3.3100
1,9572,410<3.3100<3.3100<3.3100<3.3100
2,2372,575<3.3100<3.3100<3.3100<3.3100
49,68042,660<3.3100<3.31008699.84999.9
54,49061,065<3.3100<3.310065198.810399.8
<3.3<3.3<3.3NA<3.3NA<3.3<3.3
<3.3<3.3<3.3NA<3.3NA<3.3<3.3
<3.3<3.3<3.3NA<3.3NA<3.3<3.3
  • a Preincubation was performed with either an excess of a mix of native ad and ay HBsAg subtypes or adw2 HBsAg subtype for 2 h at 37°C. Specificity represents the difference in concentrations observed before and after inhibition expressed as a percentage of the corresponding concentration measured without inhibition.