TABLE 1.

Details of fungi and results of ELISA specificity tests using MAbs GA3 and HG12

OrganismIsolate no.SourceaAbsorbance (450 nm)b
GA3HG12
Absidia corymbifera101040CBS0.0210.021
Absidia glauca2CRT0.0270.053
Absidia spinosa3CRT0.0170.063
Acremonium alternatum545.89CBS0.0020.056
Acremonium atrogriseum306.85CBS0.0280.057
Acremonium blochii424.93CBS0.0100.041
Alternaria alternata42CRT0.0070.029
Apophysomyces elegans658.93CBS0.0320.044
Aspergillus clavatus514.65CBS0.0280.034
A. fumigatusAF293SK0.0160.041
A. fumigatus181CRT0.0120.029
Aspergillus flavus91856iiiIMI0.0220.072
Aspergillus niger102.40CBS0.0260.063
A. niger121.47CBS0.0290.065
Aspergillus terreus var. terreus601.65CBS0.0370.053
Aspergillus versicolor599.65CBS0.0290.044
Aureobasidium pullulans var. pullulans771.97CBS0.0020.005
Candida albicansSC5314SB0.0160.023
Candida dubliniensis8500CBS0.0220.028
Candida glabrata4692CBS0.0180.022
Cladosporium herbarum159.59CBS0.0240.049
Cryptococcus neoformans5728CBS0.0270.047
C. neoformans7779CBS0.0330.037
Cunninghamella bertholletiae182.84CBS0.0190.062
Emericella nidulans var. nidulansA4FGSC0.0360.060
Exophiala dermatitidis153.94CBS0.0270.058
Exophiala jeanselmei var. heteromorpha657.76CBS0.0130.038
Fusarium solani224.34CBS0.0180.060
F. solani80CRT0.0280.066
F. solani var. petrophilum102256CBS0.0720.052
Fusarium verticillioides539.79CBS0.0060.044
Geotrichum capitatum327.86CBS0.0190.033
Graphium eumorphum987.73CBS1.0431.008
Graphium penicilliodes320.72CBS1.5071.518
L. fimeti129.78CBS1.2811.200
Madurella mycetomatis868.95CBS0.0130.027
Mucor plumbeus96CRT0.0270.066
Mucor racemosus93CRT0.0290.052
Paecilomyces variotii339.51CBS0.0000.002
Parascedosporium tectonae127.84CBS1.5171.462
Penicillium chrysogenum105CRT0.0290.040
Penicillium marneffei101038CBS0.0010.012
Penicillium marneffei669.95CBS0.0040.017
Penicillium purpurogenum364.48CBS0.0330.069
Petriella gutullata362.61CBS1.4561.509
Petriella setifera109039CBS1.1921.122
Petriellopsis africana311.72CBS1.1141.095
Phialophora richardsiae483.80CBS0.0280.022
Phialophora verrucosa225.97CBS0.0150.046
P. angusta254.72CBS1.0410.972
P. boydii835.96CBS1.4391.421
P. boydii100393CBS1.3011.184
P. boydii100395CBS1.1881.085
P. boydii100870CBS1.2001.071
Pseudallescheria desertorum489.72CBS1.0231.013
Pseudallescheria ellipsoidea438.72CBS1.1161.049
P. fusoidea106.53CBS1.1601.055
R. miehei360.92CBS0.0330.077
Rhizopus microsporus var. rhizopodiformis102277CBS0.0480.060
Rhizopus oryzae395.54CBS0.0860.019
Rhizopus sexualis var. sexualis209090IMI0.0100.038
Rhizopus stoloniferG1CRT0.0040.017
Saksenaea vasiformis100390CBS0.0380.074
S. aurantiacum121926CBS1.4311.243
S. aurantiacum118934CBS1.3021.271
S. apiospermum117407CBS1.0770.985
S. dehoogii117406CBS0.0260.049
S. prolificans742.96CBS0.0190.057
S. prolificans100390CBS0.0210.047
S. prolificans100391CBS0.0230.067
S. prolificans102176CBS0.0450.075
Sporothrix schenckii345.53CBS0.0340.043
Stachybotrys chartarum485.48CBS0.0160.040
Syncephalastrum racemosum155CRT0.0240.059
Trichoderma longibrachiatum446.95CBS0.0610.052
Wallemia sebi196.56CBS0.0220.046
  • a CBS, Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands; CRT, C. R. Thornton; IMI, International Mycological Institute, Egham, England; SB, S. Bates, School of Biosciences, University of Exeter; SV, S. Krappman, Institute of Microbiology and Genetics, Department of Molecular Microbiology and Genetics, Georg August University, Gottingen, Germany.

  • b Each value is the mean for replicated samples. The threshold absorbance value for detection of antigen in ELISA is ≥0.100.