TABLE 1.

Invaplex-mediated plasmid DNA transfection of mammalian cells in vitro

Transfection mediatorConcn (μg/ml)Mean % transfection with indicated plasmida
pEGFP-N1pKarp56pgWIZβ-gal
Invaplex 24105 ± 28 ± 26 ± 2
10017 ± 2*23 ± 6*19 ± 3*
50020 ± 1*32 ± 9*14 ± 2*
Invaplex 50103 ± 28 ± 16 ± 1
10024 ± 6*30 ± 6*28 ± 1*
50023 ± 2*29 ± 5*20 ± 5*
Culture medium0<1<1<1
Geneporter10 μl60 ± 6*48 ± 4*67 ± 12*
  • a The mean percent transfection from three separate experiments ± 1 standard deviation was calculated by dividing the number of cells expressing the indicated protein by the total number of cells counted (≥200). Values significantly different (P < 0.05) from the culture medium controls are indicated with an asterisk.