TABLE 4.

Detection of anthrax PA in mice injected with different doses of PA and LF by ENIAa

Toxin dose injected (μg)No. of animalsPA concn measured in blood (μg/ml)
PALF
505021.37 ± 0.42b
502521.41 ± 0.09
50521.11 ± 0.21
105040.83 ± 0.36
102540.81 ± 0.28
10521.09 ± 0.05
10020.14 ± 0.04
05020.00
02520.00
0530.00
  • a BALB/cJ mice (n = 2 to 4) were injected with different amounts of wild-type PA or LF or with premixed combinations of these toxins (all prepared in PBS) and were bled 2 h after injection. PA levels in plasma were assessed by ENIA. The samples were diluted from 1:10 to 1:1,000.

  • b The data are means ± SDs.