TABLE 3.

Detection of anthrax PA in mice injected with PA, LF, and EFa

Toxin(s)Bleeding time (h)No. of animalsPA concn in blood (μg/ml) measured by:
ENIAELISA
PA231.60 ± 0.40b2.23 ± 0.31
PA621.14 ± 1.510.94 ± 1.08
PA + LF231.23 ± 0.151.33 ± 0.08
PA + LF630.70 ± 0.101.10 ± 0.15
LF230.000.00
LF630.000.00
EF230.000.00
EF630.000.00
  • a BALB/cJ mice were injected intravenously (200 μl/vein) with 50 μg of wild-type PA, LF, or EF or with premixed combinations of these toxins (all prepared in PBS) and were bled 2 or 6 h after injection. The PA levels in plasma were assessed by the ENIA and ELISA methods. The samples were diluted from 1:10 to 1:1,000.

  • b The data are means ± SDs.