TABLE 1.

Detection of anthrax PA in mouse plasma spiked with different doses of PA, LF, and EF by ENIA and ELISAa

Panel and sample no.Toxin concn spiked (ng/ml)PA concn detected (ng/ml)
PALFEFENIAELISA
Panel I
    1100,000.00027,000.00NDb
    91,000.00020.00ND
    2400.000425.00ND
    440.00018.00ND
    64.0003.30ND
    30.4000.30ND
    50.0400.00ND
    70.0040.00ND
    80.0000.00ND
Panel II
    51,720.003,400.002,500.00
    10172.00125.0037.00
    2417.2011.000.00
    251.720.600.00
    280.170.600.00
    320.021.400.00
    47170.00200.0010.000.01
    481.702.001.500.00
    540.070.081.000.00
    45170.00165.0041.000.01
    461.701.651.200.00
    342,000.000.000.00
    39200.000.000.00
    4020.000.000.00
    192.000.000.00
    140.200.000.00
    410.020.000.00
    421,650.000.000.00
    43165.000.000.00
    20.160.000.00
    530.020.000.00
  • a Plasma from untreated mice was spiked with known concentrations of toxins and PA levels were assessed by two methods. The samples were diluted from 1:10 to 1:1,000.

  • b ND, not done.